ORGNANIZATION DETAILS (viewed 1041 times)

 

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Name:gokcentas
Country:Turkey
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Objectives/Goals
The objective of this project was to determine if plants would produce salicylic acid in response to stress.
Methods/Materials
Plants were grown for testing and stressed with fungus and physical damage, then frozen using liquid
nitrogen prior to being ground into powder. Salicylic acid was extracted with a mixture of ethanol and
acetic acid, then sonicated to further break down the cell structure to release the chemicals.
The samples were subjected to a ferric chloride test. If salicylic acid was present, there would be a purple
color change. Calibration testing indicated the ferric chloride test method could only detect salicylic acid
at levels down to 0.0001 molar concentrations. As a result, to determine if a control or a stressed sample
had more salicyclic acid than the other, a know concentration solution of salicylic acid was added to both
the control and test samples in equal and known amounts until a color change was observed in one of
them. The first one to change color would, therefore, contain more salicylic acid.
Results
The testing showed more salicylic acid present in the infected and physically wounded plants than in the
control plants in all cases. The test method didn't yield an exact quantity of salicylic acid present in the
plant material

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